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sftx  (Alomone Labs)


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    Alomone Labs sftx
    Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker <t>sFTX-3.3</t> (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.
    Sftx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sftx+3+3/sFTX-3%2E3/pmc05465225-46-27-31
    Average 90 stars, based on 1 article reviews
    sftx - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells"

    Article Title: Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells

    Journal: Molecular and cellular endocrinology

    doi: 10.1016/j.mce.2016.10.008

    Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.
    Figure Legend Snippet: Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

    Techniques Used: Cell Culture

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    Article Snippet: VSCC inhibitors (Alomone Labs, Jerusalem, Israel) were added in the following concentrations: L-type inhibitor, nifedipine (1 m); T-type inhibitor, sFTX-3.3 (200 nm); P/Q-type inhibitor, -agatoxin IVA (1 m); N-type inhibitor, -conotoxin GVIA (1 m); and R-type inhibitor, SNX-482 (100 nm).

    Article Title: Bending the MDCK cell primary cilium increases intracellular calcium.
    Article Snippet: We tested the hypothesis that the primary cilium of renal epithelia is mechanically sensitive and serves as a flow sensor in MDCK cells using differential interference contrast and fluorescence microscopy.. Bending the cilium, either by suction with a micropipette or by increasing the flow rate of perfusate, causes intracellular calcium to substantially increase as indicated by the fluorescent indicator, Fluo-4.. This calcium signal is initiated by Ca-influx through mechanically sensitive channels that probably reside in the cilium or its base.



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    Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

    Journal: Molecular and cellular endocrinology

    Article Title: Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells

    doi: 10.1016/j.mce.2016.10.008

    Figure Lengend Snippet: Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

    Article Snippet: Test substances Serotonin, dibutyryladenosine 3′,5′-cyclic monophosphate (dbcAMP), 1-methyl-3-isobutylxanthine (IBMX), chelerythrine, H-89, GR113808, zacopride, EGTA, nifedipine, mibefradil, NiCl 2 were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). ω-conotoxin and sFTX-3.3 were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Cell Culture

    Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

    Journal: Molecular and cellular endocrinology

    Article Title: Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells

    doi: 10.1016/j.mce.2016.10.008

    Figure Lengend Snippet: Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

    Article Snippet: Serotonin, dibutyryladenosine 3′,5′-cyclic monophosphate (dbcAMP), 1-methyl-3-isobutylxanthine (IBMX), chelerythrine, H-89, GR113808, zacopride, EGTA, nifedipine, mibefradil, NiCl 2 were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). ω-conotoxin and sFTX-3.3 were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Cell Culture